Estandarización de un protocolo para la desinfección y el establecimiento in vitro de segmentos nodales de Cantuaz buxifolia Juss.
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Authors
Quispe Medrano, Celia Leticia
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Abstract
El objetivo del presente estudio fue de establecer una base de datos referencial de las constantes fisiológicas (frecuencia cardiaca: FC, frecuencia respiratoria: FR, temperatura rectal: T°R y temperatura de la piel: T°P) por sexo, de los Caballos Peruano de Paso en la costa central peruana, durante los estados estático (reposo) y dinámico (caminata y al cabestro). Los datos se registraron en 31 caballos de la Unidad Ecuestre de la Universidad Nacional Agraria La Molina, considerando 03 sexos (hembras: 18; machos enteros: 6 y machos castrados: 7). La edad de los animales oscilaban entre 2 y 19 años, y todos se encontraban en buen estado de salud. Para el registro de las constantes fisiológicas se utilizó el estetoscopio, termómetros y un monitor de señales (MOSIVILLe). Los datos obtenidos fueron analizados mediante estadística descriptiva y para las comparaciones se usaron “t” de student y U de Mann-Whitney. Se encontró que la FC fue de 40 ± 6.01 lpm durante el reposo, 63.94 ± 11.63 lpm para la caminata y 106.8 ± 14.09 lpm para el cabestro; las frecuencias respiratorias resultaron ser 25.32 ± 10.49 rpm en reposo, 51.56 ± 8.40 rpm durante la caminata y 92.77 ± 7.86 rpm durante el cabestro. Al comparar las constantes fisiológicas por sexo se aprecian bastantes similitudes con respecto a la temperatura, sin embargo, se encontraron diferencias (P > 0.05) con respecto a la FC y FR en el estado estático, mas no en el dinámico, donde los machos castrados presentaron FC y FR más bajos que los machos enteros y las hembras. En cuanto a la T°P no se encontraron diferencias entre los grupos, pero si en la T°R, donde los machos enteros tienen una temperatura más baja que los castrados.
The objective of this study was to establish a reference database of physiological constants (heart rate: HR, respiratory rate: RR, rectal temperature: RT, and skin temperature: ST) by sex for Peruvian Paso horses on the central coast of Peru during static (rest) and dynamic (walking and leading) states. The data were recorded in 31 horses from the Equestrian Unit of the National Agrarian University of La Molina, considering three sexes (females: 18; entire males: 6; and castrated males: 7). The animals ranged in age from 2 to 19 years and were all in good health. A stethoscope, thermometers, and a signal monitor (MOSIVILLe) were used to record physiological constants. The data obtained were analyzed using descriptive statistics, and Student's ttest and Mann-Whitney U test were used for comparisons. HR was found to be 40 ± 6.01 bpm at rest, 63.94 ± 11.63 bpm during walking, and 106.8 ± 14.09 bpm during halter walking. Respiratory rates were 25.32 ± 10.49 rpm at rest, 51.56 ± 8.40 rpm during walking, and 92.77 ± 7.86 rpm during halter use. When comparing physiological constants by sex, there were many similarities with regard to temperature; however, differences (P > 0.05) were found with regard to HR and RR in the static state, but not in the dynamic state, where castrated males had lower HR and RR than entire males and females. Regarding T°P, no differences were found between groups, but differences were found in T°R, where the entire males had a lower temperature than the castrated males.
The objective of this study was to establish a reference database of physiological constants (heart rate: HR, respiratory rate: RR, rectal temperature: RT, and skin temperature: ST) by sex for Peruvian Paso horses on the central coast of Peru during static (rest) and dynamic (walking and leading) states. The data were recorded in 31 horses from the Equestrian Unit of the National Agrarian University of La Molina, considering three sexes (females: 18; entire males: 6; and castrated males: 7). The animals ranged in age from 2 to 19 years and were all in good health. A stethoscope, thermometers, and a signal monitor (MOSIVILLe) were used to record physiological constants. The data obtained were analyzed using descriptive statistics, and Student's ttest and Mann-Whitney U test were used for comparisons. HR was found to be 40 ± 6.01 bpm at rest, 63.94 ± 11.63 bpm during walking, and 106.8 ± 14.09 bpm during halter walking. Respiratory rates were 25.32 ± 10.49 rpm at rest, 51.56 ± 8.40 rpm during walking, and 92.77 ± 7.86 rpm during halter use. When comparing physiological constants by sex, there were many similarities with regard to temperature; however, differences (P > 0.05) were found with regard to HR and RR in the static state, but not in the dynamic state, where castrated males had lower HR and RR than entire males and females. Regarding T°P, no differences were found between groups, but differences were found in T°R, where the entire males had a lower temperature than the castrated males.
Description
Universidad Nacional Agraria La Molina. Facultad de Agronomía. Departamento
Académico de Horticultura
Keywords
Micropropagación
Citation
Date
2025
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